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The hypothalamo-neurohypophysial complex of Ailia coila is well demonstrated with the help of in situ staining procedure. Both pars magnocellularis and pars parvocellularis components of the nucleus preopticus contribute to the formation of the right and the left main neurosecretory tracts. Anterior one third of these tracts are loosely set and posteriorly they became more compact. From the posterior two thirds of the main tracts several pairs of lateral tracts were given off which join at the midline to form the paired median tracts. The median and the main tracts jointly enter the pituitary as the common tract. The common tract on entering the pituitary often divides into two or more branches and enter the pars intermedia independently. The rostral pars distalis is least innervated by the neurosecretory axons. Since the proximal pars distalis has varying amount of AF-positive cells, and the pars intermedia has the bulk of the neurosecretory axons both these regions are stained dark in the in situ preparations. Bulk preparations provide a clear topographic picture of the entire neurosecretory system, which is very difficult to visualise in tissue sections and in their reconstructions. 相似文献
545.
To determine the separate spermatogenic actions of FSH and testosterone, adult male lizards Hemidactylus flaviviridis with recrudescent testes were administered the non-steroidal antiandrogen flutamide either alone or in combination with FSH or testosterone, and the histology and histochemistry of the testes and ductus epididymides were studied. Flutamide-treated animals displayed a marked hypertrophy of Leydig cells. A few spermatids were also seen in testis of more than half the animals treated with flutamide. Flutamide also produced a significant increase of primary spermatocytes; no spermatids were observed in controls. A significant inhibition of spermatogenesis was noted in lizards treated either with testosterone alone or in combination with flutamide. Ovine FSH treatment caused a significant stimulation of spermatogenesis, as indicated by the increase of primary and secondary spermatocytes and the transformation of secondary spermatocytes into spermatids or, in a few cases, into spermatozoa. A considerable depletion of sudanophilic lipid and moderate delta 5-3 beta-hydroxysteroid dehydrogenase activity was noted in the Leydig cells of FSH-treated animals indicating enhanced steroidogenesis. Similar results were obtained when lizards were treated with flutamide + FSH. The effects of simultaneous treatment of flutamide with FSH or testosterone on ductus epididymidis revealed that flutamide markedly inhibited the epithelial cell height and lumen diameter with a loss of luminal content when compared to FSH or testosterone-treated lizards. 相似文献